In order to master the types and antibiotic resistance trend of the main pathogen of P.crocea in Ningbo City, Zhejiang Province in recent years, the infected fishes were collected from the main breeding sites of P.crocea in Ningbo from 2020 to 2022 for isolation and identification of pathogens.In the meantime, the minimum inhibitory concentration (MIC) of antibiotics (enrofloxacin, neomycin sulfate, thiamphenicol, florfenicol, doxycycline hydrochloride, fluoroquine, sulfamethoxazole sodium, sulfamethoxazole + trimethoprim) were determined to compare the susceptibility of each pathogen to the drug.The results showed that 7 species and 58 strains of pathogens were isolated from P.crocea in recent three years.The main pathogens were Pseudomonas plecoglossicida, Vibrio harveyi and Nocardia seriolea,accounting for 74.1% of the total bacterial strains. V.harveyi and N.seriolae were isolated every year. The isolated strains were 100% sensitive to doxycycline hydrochloride.The susceptibility of all strains to this drug was distributed at MIC ≤ 4.00 mg/L and the drug resistance rate was 0%.In comparison,the drug tolerance concentration of pathogens to florfenicol and sulfonamides was relatively high, especially the MIC of sulfonamides was generally between 64.00-1 024.00 mg/L. In addition,from the annual change in drug resistance of pathogen, it could be seen the drug resistance of N.seriolae was increasing year by year. Therefore,although the pathogens were generally sensitive to doxycycline hydrochloride,neomycin sulfate and enrofloxacin,which could be used as the preferred drug for controlling most pathogens in P.crocea,the dose and course must be determined strictly according to the results of drug sensitivity test and in combination with pharmacokinetics and pharmacodynamics, in order to avoid the emergence of drug-resistant strains as much as possible.